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. 1980 Dec 11;8(23):5541-9.
doi: 10.1093/nar/8.23.5541.

A subcloning strategy for DNA sequence analysis

A M Frischauf, H Garoff, H Lehrach
Free PMC article

A subcloning strategy for DNA sequence analysis

A M Frischauf et al. Nucleic Acids Res. .
Free PMC article

Abstract

We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced directly without further strand- or fragment separation steps.

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