[Participation of cyclic nucleotides in the mechanism of regulation of the tissue pool of coenzyme A and the acetylizing capacity of rats]

Abstract

cAMP and dibutyryl-cAMP were shown to increase the pool of CoA (or all the pantothenic coenzymes) in rat liver tissue, especially after treatment of the animals with 4-phosphopantothenate but not with pantethine. As shown by experiment with inhibitors of protein synthesis (actinomycin D, actidion, chloramphenicol) immediate c-AMP-dependent stimulation of the biogenesis of pantothenic coenzymes occurred rather from their precursors than due to induction of enzymes, participating in the biogenesis, or to destruction of the pantothenic coenzymes. Experiments with chloramphenicol exhibited that cAMP regulation of the biogenesis of pantothenic coenzymes in mitochondria was responsible mainly for the cellular pool of CoA and, partly, for the acetylation of para-aminobenzoic acid in tissues.