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. 1981 Jan 10;9(1):85-93.
doi: 10.1093/nar/9.1.85.

Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases

L Ferretti, V Sgaramella

Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases

L Ferretti et al. Nucleic Acids Res. .

Abstract

The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI restriction endonucleases has been investigated by electron microscopy analysis. The extent of joining is maximal at 4 degrees and decreases with increasing temperatures following sigmoid-like curves. The temperature at which 50% of the maximal reaction is still observable increases going from DNA termini without single-stranded overlaps (produced by HaeIII) to termini with four nucleotides overlap, composed only by two A and two T (produced by EcoRI) to termini with four nucleotide overlap, composed by A, T, G and C (produced by PstI).

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