Localization of receptor-human chorionic gonadotropin complex to the surface of the luteal and interstitial glandular cells in the pseudopregnant rat ovaries with the peroxidase-antiperoxidase (PAP) complex method

Abstract

Receptor-bound hCG was localized in pseudopregnant rat ovarian cells at semiultrastructural level with the peroxidase-antiperoxidase (PAP) complex method. The animals received 2, 6, 12 or 24 h prior to killing a single intravenous injection of hCG and the hormone was localized in the 5-micrometer paraffin sections and in the 1-micrometer epon sections using the pre-embedding technique. The peroxidase staining localized to the periphery of the luteal and interstitial glandular cells. No significant staining occurred in the intracellular structures of the cells during the 24-h observation period. However, the appearance of staining in the subplasmalemmal structures can not be excluded. These results are compatible with the previous observations that the receptor-hCG complexes are primarily formed at the surface of the luteal and interstitial glandular cells.