Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Mar;31(3):1062-70.
doi: 10.1128/iai.31.3.1062-1070.1981.

Assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera

R Eberle, R J Courtney

Assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera

R Eberle et al. Infect Immun. 1981 Mar.

Abstract

A reliable and reproducible method for determining specific reactivity to herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) in human sera has been developed. Human sera were used to immunoprecipitate HSV-specific glycoprotein antigens from both HSV-1- and HSV-2-infected cell extracts. The viral glycoproteins precipitated from these extracts were then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to detect specific reactivity of the sera with distinct type-specific antigens of HSV-1, HSV-2, or both as well as with type-common glycoprotein antigens. By examining a large number of human sera, this method was found to be more reliable than the standard microneutralization test in discriminating between single-positive (positive for HSV-1 or HSV-2) and double-positive (positive for HSV-1 and HSV-2) sera.

PubMed Disclaimer

References

    1. J Immunol. 1967 May;98(5):941-7 - PubMed
    1. J Virol. 1980 Dec;36(3):665-75 - PubMed
    1. J Immunol. 1969 Apr;102(4):956-62 - PubMed
    1. J Immunol. 1970 Mar;104(3):599-606 - PubMed
    1. J Obstet Gynaecol Br Commonw. 1971 Nov;78(11):1031-8 - PubMed

Publication types

LinkOut - more resources