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. 1980;98(3):233-42.
doi: 10.1007/BF00410787.

Autoradiographic observations on the distribution and metabolism of N'-/14C/nitrosonornicotine in mice

E Brittebo, H Tjälve

Autoradiographic observations on the distribution and metabolism of N'-/14C/nitrosonornicotine in mice

E Brittebo et al. J Cancer Res Clin Oncol. 1980.

Abstract

The fate of the weak base N'-nitrosonornicotine (2'-14C-labeled) has been studied in mice. Whole-body autoradiography showed that the radioactivity was rapidly distributed throughout the body, which probably reflects an ability of the non-protonated compound to freely pass biological membranes and distribute evenly in the intra- and extracellular tissue water. Metabolites which were firmly bound to tissue macromolecules - retained throughout the observational period (24 h) - were present in the tracheobronchial and nasal mucosa, the liver, the submaxillary and sublingual salivary glands and the esophagus. Radioactivity in the lacrimal gland, the gastrointestinal contents, the urinary bladder, and gallbladder seems to be related to excretion of the substance and/or its metabolites. A binding to the melanin in the eye and hair was observed in vivo and in vitro. Experiments with mice pretreated with diethyldithiocarbamate and nialamide showed that these substances partially inhibited the metabolism of N'-/14C/nitrosonornicotine. CO2 was not recovered in the breath during the 4 h following the administration of N'-/14C/nitrosonornicotine.

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References

    1. Abanobi SE, Popp JA, Chang SK, Harrington GW, Lotlikar PD, Hadjiolov D, Levitt M, Rajalakshmi S, Sarma DSR (1977) Inhibition of dimethylnitrosamine-induced strand breaks in liver DNA and liver cell necrosis by diethylthiocarbamate. J Natl Cancer Inst 58:263–271 - PubMed
    1. Appelgren L-E, Hansson E, Schmiterlöw CG (1963) Localization of radioactivity in the superior cervical ganglion of cats following injection of C14-labelled nicotine. Acta Phhsiol Scand 59:330–336 - PubMed
    1. Boyland E, Roe FJC, Gorrod JW (1964) Induction of pulmonary tumours in mice by nitrosonornicotine, a possible constituent of tobacco smoke. Nature 202:1126 - PubMed
    1. Chen CB, Hecht SS, Hoffmann D (1978) Metabolic α-hydroxylation of the tobacco-specific carcinogen, N′-nitrosonornicotine. Cancer Res 38:3639–3645 - PubMed
    1. Cottrell RC, Young PJ, Herod IA, Lake BG, Phillips JC, Gangolli SD (1977) Preliminary studies of the metabolism of N-nitroso/2,5-14C/pyrrolidine in the rat. Biochem Soc Trans 5:1011–1013 - PubMed

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