Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Apr;89(1):162-8.
doi: 10.1083/jcb.89.1.162.

Transport of newly synthesized vesicular stomatitis viral glycoprotein to purified Golgi membranes

J E Rothman, E Fries

Transport of newly synthesized vesicular stomatitis viral glycoprotein to purified Golgi membranes

J E Rothman et al. J Cell Biol. 1981 Apr.

Abstract

In a previous communication we reported that the newly synthesized membrane glycoprotein of vesicular stomatitis virus could be transported in crude extracts of CHO cells from endoplasmic reticulum-derived membranes to membranes of the Golgi complex. This conclusion was an indirect one, based on the terminal glycosylation of this glycoprotein, a reaction that was dependent upon a Golgi-specific enzyme, UDP-GlcNAc transferase I. We show here that the Golgi fraction of rat liver will substitute for members of CHO cells as a source of transferase I in this reaction. The use of highly purified fractions of liver Golgi membranes, coupled with the ability to recover these membranes from incubations, has now permitted a direct demonstration of net transport of G protein to these heterologous Golgi membranes. This transport reaction is specific, in that the smooth endoplasmic reticulum fraction will not substitute for the Golgi fraction, is quantitatively significant, involving at least 30% of the viral glycoprotein, and is sustained only in the presence of both ATP and a soluble, cytosol fraction of liver cells.

PubMed Disclaimer

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Cell Biol. 1974 Nov;63(2 Pt 1):357-63 - PubMed
    1. Methods Enzymol. 1974;31:3-6 - PubMed
    1. Methods Enzymol. 1974;31:90-102 - PubMed
    1. J Biol Chem. 1975 Feb 25;250(4):1434-44 - PubMed

Publication types