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. 1981 Mar 25;9(6):1301-9.
doi: 10.1093/nar/9.6.1301.

Characterisation of the genes for ribosomal RNA in flax

P B Goldsbrough, C A Cullis
Free PMC article

Characterisation of the genes for ribosomal RNA in flax

P B Goldsbrough et al. Nucleic Acids Res. .
Free PMC article

Abstract

DNA coding for the 18S and 25S rRNAs in flax (Linum usitatissimum) has been purified and is arranged in tandem arrays with a repeat length of 8.6 kb. There is no detectable variation in the size of this repeat unit. Single repeat units have been cloned in the plasmid pAT 153. The coding sequences for the 25S, 18s and 5.85 rRNAs have been localised by hybridisation. The cloned rDNA has been used to compare two genotrophs, L1 ad S1, where the number of rRNA cistrons has been altered by growth under different environmental conditions. In terms of the size of the repeat unit and the position of a number of restriction enzyme sites the rDNAs from L1 and S1 were identical.

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