Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Feb;78(2):943-7.
doi: 10.1073/pnas.78.2.943.

Simian virus 40 tandem repeated sequences as an element of the early promoter

Simian virus 40 tandem repeated sequences as an element of the early promoter

P Gruss et al. Proc Natl Acad Sci U S A. 1981 Feb.

Abstract

On the late side of the simian virus 40 (SV40) DNA replication origin are several sets of tandem repeated sequences, the largest of which is 72 base pairs long. The role of these sequences was examined through construction of deletion mutants of SV40. A mutant from which one of the 72-base-pair repeated units was removed is viable upon transfection of monkey kidney cells with viral DNA. Extension of this deletion into the second repeated unit, however, leads to nonviability, as recognized by the absence of early transcription and of tumor antigen production. These observations indicate that the 72-base-pair repeated sequences form an essential element in the early viral transcriptional promoter and explain the inability of such a deleted genome to complement an early temperature-sensitive mutant of SV40, tsA, as well as the failure to replicate its DNA. In a parallel experiment it was found that the extended deletion mutant was also unable to complement a late temperature-sensitive mutant of SV40, tsB. This suggests that the extended mutant is also defective in DNA replication or late transcription (or both).

PubMed Disclaimer

References

    1. Science. 1979 Oct 26;206(4417):456-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4317-21 - PubMed
    1. Cell. 1980 Jan;19(1):13-25 - PubMed
    1. Cell. 1980 Jan;19(1):27-35 - PubMed
    1. Cell. 1980 Mar;19(3):717-28 - PubMed