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. 1981 Mar;78(3):1746-50.
doi: 10.1073/pnas.78.3.1746.

Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane

J E Bergmann, K T Tokuyasu, S J Singer

Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane

J E Bergmann et al. Proc Natl Acad Sci U S A. 1981 Mar.

Abstract

The intracellular pathway of biogenesis of the vesicular stomatitis virus transmembrane glycoprotein was investigated in situ by using indirect immunofluorescence of whole infected Chinese hamster ovary cells and immunoelectron microscopy of ultrathin frozen sections of infected cells. Transport of the glycoprotein was synchronized by using the temperature-sensitive virus mutant Orsay-45 and a temperature shift-down protocol. Sequential appearance of the glycoprotein in the rough endoplasmic reticulum, Golgi apparatus, and plasmalemma was demonstrated. The potential of this system for further studies is discussed.

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