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. 1981 Mar;13(2):197-202.
doi: 10.1016/0378-1119(81)90008-1.

SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen

G Chu, P A Sharp

SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen

G Chu et al. Gene. 1981 Mar.

Abstract

A modification of the Graham and Van der Eb (1974) DNA-calcium phosphate coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA. The transfection is done in suspension after detachment of cells by trypsin digestion. Transfection efficiency was measured by staining cells for the presence of SV40 T-antigen by indirect immunofluorescence and by assaying for the presence of SV40 early message by the Berk and Sharp (1978) technique.

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