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. 1981 Jul;147(1):262-6.
doi: 10.1128/jb.147.1.262-266.1981.

Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B

H P Blaschek, M Solberg

Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B

H P Blaschek et al. J Bacteriol. 1981 Jul.

Abstract

Clostridium perfringens strain ATCC 3626B was cured of caseinase activity at a high frequency after treatment with acriflavine dye (2.5%) or elevated temperature growth (9.1%). Caseinase-negative isolates retained the larger (9.4 megadaltons) pHB102 cryptic plasmid, but were missing the smaller (2.1 megadaltons) pHB101 plasmid present in the caseinase-positive wild-type strain. Dye-buoyant density-gradient centrifugation at 4 or 15 degrees C revealed that the pHB101 and pHB102 plasmids are temperature labile and easily converted into the nicked non-supercoiled or linear state.

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