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. 1981 May;38(2):671-9.
doi: 10.1128/JVI.38.2.671-679.1981.

Integrated simian virus 40 DNA: nucleotide sequences at cell-virus recombinant junctions

J R Stringer

Integrated simian virus 40 DNA: nucleotide sequences at cell-virus recombinant junctions

J R Stringer. J Virol. 1981 May.

Abstract

DNA fragments containing the integrated viral DNA present in the simian virus 40 (SV40)-transformed rat cell lines SVRE9 and SVRE17 were cloned in procaryotic vectors, and the DNA sequences linking SV40 and cell DNA were determined. Comparison of the DNA sequences at the SV40-cell junctions in SVRE9 and SVRE17 cells with those of a previously characterized viral insertion from SV14B cells shows that no specific viral or cellular sequences occur at SV40-cell junctions and that the cellular DNA sequences adjacent to integrated SV40 DNA do not display the direct repeat structure characteristic of transposons and retrovirus proviruses.

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References

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