Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein
- PMID: 6264459
- PMCID: PMC319279
- DOI: 10.1073/pnas.78.4.2043
Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein
Abstract
The polypeptide product of the lon (capR) gene was identified and partially purified from bacterial strains homozygous for the capR(+) or capR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR(+) and capR9 proteins bind DNA.
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