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. 1981;22(1):32-8.
doi: 10.1186/BF03547203.

Detection of porcine rotavirus by EM, ELISA and CIET

J Askaa, B Bloch

Detection of porcine rotavirus by EM, ELISA and CIET

J Askaa et al. Acta Vet Scand. 1981.

Abstract
in English, Norwegian

Feces samples from swine-herds with severe problems of neonatal diarrhoea, 3 weeks scours or early weaning diarrhoea were examined for porcine rotavirus using three techniques: EM, ELISA and CIET. Infection with rotavirus was found in about one third of the feces samples, representing more than half of the examined swine-herds. EM and ELISA revealed nearly all the samples found to be positive, while CIET as used here detected only half of them.

Fæcesprøver fra svinebesætninger med længere varende problemer med neonatal, tre ugers eller tidlig fravænnings diarré blev undersøgt for tilstedevserelse af porcin rotavirus ved hjælp af elektronmikroskopi (EM), “enzyme linked immunosorbent assay” (ELISA), og modstrømselektroforese (CIET). Rotavirus blev påvist i omkring en trediedel af fæcesprøverne og i mere end halvdelen af de undersøgte svinebesætninger. I næsten alle rotavirus-positive prøver fandtes virus ved hjælp af EM og ELISA, mens det kun var muligt at påvise virus i halvdelen af alle rotavirus-positive prøver ved hjælp af CIET.

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