Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Aug;147(2):297-304.
doi: 10.1128/jb.147.2.297-304.1981.

Identification of the tetracycline resistance promoter and repressor in transposon Tn10

L V Wray Jr, R A Jorgensen, W S Reznikoff

Identification of the tetracycline resistance promoter and repressor in transposon Tn10

L V Wray Jr et al. J Bacteriol. 1981 Aug.

Abstract

The structural and regulatory functions encoding tetracycline resistance in transposon Tn10 lie within a 2,700-base pair region. Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, we located the promoter controlling the expression of tetracycline resistance. These various sequences conferred altered levels of tetracycline resistance. Plasmids containing deletions of a 695-base pair HincII fragment were constitutive and showed the loss of a 23,000-dalton tetracycline-inducible polypeptide, thus identifying the repressor and the location of its gene.

PubMed Disclaimer

References

    1. J Bacteriol. 1966 Feb;91(2):628-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932-6 - PubMed
    1. J Mol Biol. 1970 Oct 14;53(1):159-62 - PubMed
    1. J Mol Biol. 1971 Feb 28;56(1):53-62 - PubMed
    1. Mol Gen Genet. 1972;117(4):349-66 - PubMed

Publication types

LinkOut - more resources