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. 1981 Jul;3(1):37-43.
doi: 10.1016/0166-0934(81)90020-3.

Cloning of hepatitis A virus genome

K von der Helm, E L Winnacker, F Deinhardt, G Frösner, V Gauss-Müller, B Bayerl, R Scheid, G Siegl

Cloning of hepatitis A virus genome

K von der Helm et al. J Virol Methods. 1981 Jul.

Abstract

Hepatitis A virus (HAV) was highly purified from faeces. The genomic RNA was transcribed to cDNA and this DNA was then cloned into plasmid pBR 322 at the Pst I site, and clones were selected in presence of tetracycline. Most clones contained inserts which hybridized to HAV-specific RNA isolated from HAV-infected cell cultures derived from a human hepatocellular carcinoma. Two clones expressed low amounts of viral antigens.

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