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. 1981;68(3-4):197-202.
doi: 10.1007/BF01314572.

Biochemical characterization of simian foamy virus type i

F Cavalieri, A Rhodes-Feuillette, A B Benzair, R Emanoïl-Ravicovitch, J Peries

Biochemical characterization of simian foamy virus type i

F Cavalieri et al. Arch Virol. 1981.

Abstract

Simian syncitium-forming ("foamy") virus type I (SFV1) was characterized biochemically. RNA was extracted from purified virus either with 0.1 per cent SDS or by the standard phenol-chloroform method. By both techniques a main component of 65-70S was found. Denaturation of the 65-70S RNA by heat resulted in a shift of the sedimentation coefficient mainly to a 30-35S component. Electrophoresis on a composite polyacrylamide gel demonstrated the existence of three minor RNA's: 8S, 5S and 4S respectively. PAGE-SDS analysis of disrupted purified virions enabled the separate migration of five viral proteins and the identification of two main proteins: a 30 kd polypeptide and a 70 kd polypeptide.

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