[In vivo restriction of Escherichia coli-transforming DNA by endonuclease R.M.EcoRI]
- PMID: 6268481
[In vivo restriction of Escherichia coli-transforming DNA by endonuclease R.M.EcoRI]
Abstract
Transformation of Escherichia coli K-12 for various chromosomal markers was accomplished by using AB1157 recBC+ strain as a recipient. The yield of transformants was reduced 10-fold, as compared with that obtained in JC7623 recBC sbcB recipient. Elimination of transformation has been obtained for arg, pro, his markers in AB1157 (pSA14) harbouring the R.M.EcoRI coding plasmid. Production of restriction endonuclease in this strain did not affect the efficiency of transformation for thr, leu markers. The presence of pSA25 which is isogenic to pSA14 but devoid of R.M.EcoRI genes has been irrelevant to transformation for leu, arg, pro, his, thr markers. Correlation between the restriction of transformed markers in vivo and in vitro is discussed.
Similar articles
-
[Plasmid recombination stimulated by restriction endonuclease EcoRI in vivo: formation of recombinant plasmids in recA+-cells of E. coli].Mol Gen Mikrobiol Virusol. 1985 Apr;(4):15-21. Mol Gen Mikrobiol Virusol. 1985. PMID: 3025697 Russian.
-
[Characteristics of RecA-independent recombination of plasmids in E. coli cells producing restriction endonuclease EcoRI].Mol Gen Mikrobiol Virusol. 1985 May;(5):13-9. Mol Gen Mikrobiol Virusol. 1985. PMID: 3025706 Russian.
-
[Localization of the genes coding for the specific modification-restriction system in the E. coli tF strain].Vet Med Nauki. 1987;24(8):9-12. Vet Med Nauki. 1987. PMID: 2830710 Bulgarian.
-
[Plasmids and DNA cloning in streptomycetes].Mikrobiol Zh (1978). 1983 Mar-Apr;45(2):96-107. Mikrobiol Zh (1978). 1983. PMID: 6306414 Review. Russian. No abstract available.
-
[Restriction enzymes. II. Mechanisms of action of type I and III restriction-modyfication systems (author's transl)].Postepy Biochem. 1980;26(3):369-87. Postepy Biochem. 1980. PMID: 6259636 Review. Polish. No abstract available.