Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Jul 10;9(13):2999-3014.
doi: 10.1093/nar/9.13.2999.

Molecular cloning of the complete Epstein-Barr virus genome as a set of overlapping restriction endonuclease fragments

J R Arrand, L Rymo, J E Walsh, E Björck, T Lindahl, B E Griffin

Molecular cloning of the complete Epstein-Barr virus genome as a set of overlapping restriction endonuclease fragments

J R Arrand et al. Nucleic Acids Res. .

Abstract

A complete collection of fragments of Epstein-Barr virus DNA, obtained by cleavage with restriction endonuclease Eco RI, has been cloned. Fourteen different internal fragments of the virus genome, derived from linear virion DNA of the B95-8 strain, and sequences corresponding to the terminal regions of virion DNA, derived from intracellular circular EBV DNA isolated from 895-8 cells, were cloned. Sizes of fragments were determined by agarose gel electrophoresis and their sum leads to an estimated molecular weight of 110 x 10(6) for virion DNA. Large Eco RI DNA fragments of special interest were also cloned in cosmids using another source of EBV DNA, that is, to circular viral DNA derived from Raji cells. In order to provide a set of overlapping sequences, all the 29 internal Bam HI fragments of B95-8 virion DNA were cloned in pBR322. The map location within the viral genome of each cloned DNA fragment was identified by hybridizing to blots of virion DNA cleaved with several different restriction endonucleases.

PubMed Disclaimer

References

    1. J Mol Biol. 1967 Jun 28;26(3):373-87 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 May;57(5):1302-9 - PubMed
    1. J Bacteriol. 1972 May;110(2):667-76 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Jan;70(1):190-4 - PubMed
    1. J Mol Biol. 1975 Jan 25;91(3):315-28 - PubMed

Publication types

Associated data