Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981;182(2):183-8.
doi: 10.1007/BF00269656.

An IS4-encoded protein is synthesized in minicells

K Trinks, P Habermann, K Beyreuther, P Starlinger, R Ehring

An IS4-encoded protein is synthesized in minicells

K Trinks et al. Mol Gen Genet. 1981.

Abstract

A protein of Mr 47,000 is synthesized in Escherichia coli minicells, when these harbor a multicopy plasmid carrying IS4 in either orientation and between different flanking sequences. The protein corresponds to the sequence predicted from the known DNA sequence of IS4, as shown by partial N-terminal radiolabel protein sequence analysis. Its apparent molecular weight, however, as determined from its electrophoretic mobility in SDS polyacrylamide gels, is smaller than predicted. When compared with other plasmid-encoded proteins, the IS4-encoded protein is synthesized in minicells in small amounts. Its synthesis has not been detected in a DNA-dependent cell-free system.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7176-80 - PubMed
    1. Biochem Soc Trans. 1980 Dec;8(6):675-6 - PubMed
    1. Nucleic Acids Res. 1979 Mar;6(3):1111-22 - PubMed
    1. Mol Gen Genet. 1971;112(1):14-27 - PubMed

Publication types

Substances

LinkOut - more resources