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. 1981;69(3-4):307-10.
doi: 10.1007/BF01317347.

Nucleoside triphosphate phosphohydrolase activities in African swine fever virus

J Kuznar, M L Salas, E Viñuela

Nucleoside triphosphate phosphohydrolase activities in African swine fever virus

J Kuznar et al. Arch Virol. 1981.

Abstract

African swine fever virus contains nucleoside triphosphate phosphohydrolase activity which releases 32P phosphate from gamma-32P ATP at a rate of about 1 mumol/h mg of virus protein. The hydrolase activity is slightly stimulated by adding nucleic acids to the reaction mixture and under conditions of RNA synthesis. A study of the rate of ATP hydrolysis at different concentrations of ATP suggests the existence of two phosphohydrolase activities with apparent Km values of about 0.04 and 1 mM.

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