doi: 10.1016/0378-1119(81)90126-8.
Modification of the bacteriophage vector M13mp2: introduction of new restriction sites for cloning
- PMID: 6271640
- DOI: 10.1016/0378-1119(81)90126-8
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Modification of the bacteriophage vector M13mp2: introduction of new restriction sites for cloning
Gene.
1981 Nov.
Abstract
The construction of two new derivatives of the bacteriophage cloning vector M13mp2 is described. One derivative, mWJ22, contains a new HindIII site while the other, mWJ43, contains a new BamHI site. These new sites were both introduced at the EcoRI site at amino acid five of the 145 amino acid-long fragment of Escherichia coli beta-galactosidase within the phage. The new restriction sites do not disrupt the blue color detection system of M13mp2; therefore insertion of cloned fragments results in colorless plaques on indicator plates for the new derivatives.
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