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. 1981;182(3):520-2.
doi: 10.1007/BF00293948.

pED100, a conjugative F plasmid derivative without insertion sequences

N Willetts, D Johnson

pED100, a conjugative F plasmid derivative without insertion sequences

N Willetts et al. Mol Gen Genet. 1981.

Abstract

The largest HindIII fragment of F includes the entire replication and transfer regions, and its circularisation with ligase gave the conjugative plasmid pED100. This plasmid, which contains none of the F insertion sequences, was essentially unable to mobilise the E. coli chromosome or to give integrative suppression of a dnaA strain.

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