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. 1981 Sep 11;9(17):4355-66.
doi: 10.1093/nar/9.17.4355.

The nucleotide sequence of the replication origin of plasmid NTP1

The nucleotide sequence of the replication origin of plasmid NTP1

J N Grindley et al. Nucleic Acids Res. .

Abstract

The sequence of the DNA of the origin region of NTP1 has been obtained. Analysis of the sequence indicates that: (1) there is great sequence homology in the DNA upstream from the origin in NTP1, ColE1, CLODF13, PBR345 AND PBR322; (2) only seven base pairs of NTP1 are identical with the sequence downstream from the origin in ColE1, although some homology exists for 140 bases downstream; (3) two ten base pair direct repeats are present in NTP1 which are also conserved in all four plasmids named above; (4) probably no polypeptide greater than fifteen amino acids in length is encoded by the NTP1 origin region, since no single open reading frame is conserved in all five plasmids.

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