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. 1981 Sep 25;9(18):4525-36.
doi: 10.1093/nar/9.18.4525.

Two new restriction endonucleases from Proteus vulgaris

T R Gingeras, L Greenough, I Schildkraut, R J Roberts

Two new restriction endonucleases from Proteus vulgaris

T R Gingeras et al. Nucleic Acids Res. .

Abstract

Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.

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