Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Nov 20;214(4523):916-9.
doi: 10.1126/science.6272391.

Cloned poliovirus complementary DNA is infectious in mammalian cells

V R Racaniello, D Baltimore

Cloned poliovirus complementary DNA is infectious in mammalian cells

V R Racaniello et al. Science. .

Abstract

A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.

PubMed Disclaimer

Publication types

LinkOut - more resources