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. 1976 Oct 29;49(3):245-51.
doi: 10.1007/BF00492380.

Histochemical evidence for sulfomucins at the surface of Hela cells

M Mareel, C Dragonetti, G De Bruyne

Histochemical evidence for sulfomucins at the surface of Hela cells

M Mareel et al. Histochemistry. .

Abstract

The nature of the negatively charged groups present at the surface of HeLa cells was further investigated. Therefore we applied a series of light microscopic staining techniques, widely used for the demonstration of epithelial mucosubstances on tissue sections, to HeLa cells from suspension cultures. Our histochemical findings confirmed the presence of carboxylated substances at the surface of these cells. Furthermore we obtained conclusive evidence for the presence of sulfated molecules. Both substances seem to be closely related to epithelial sialomucins and sulfomucins.

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