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. 1981 Oct 10;9(19):5175-84.
doi: 10.1093/nar/9.19.5175.

Lac repressor - lac operator interaction. Circular dichroism study

F Culard, J C Maurizot

Lac repressor - lac operator interaction. Circular dichroism study

F Culard et al. Nucleic Acids Res. .

Abstract

The interaction between lac repressor and a small operator DNA fragment have been examined by circular dichroism spectroscopy. The binding of lac repressor on the operator induces a conformation change of the DNA which is different from that observed upon non specific binding on non operator DNA. The CD titration curve indicates that the stoechiometry of interaction is complex. A two operators-one repressor complex was found. This result was confirmed by a gel filtration experiment.

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References

    1. J Biol Chem. 1980 Nov 10;255(21):10107-14 - PubMed
    1. Nucleic Acids Res. 1978 Jul;5(7):2373-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3576-80 - PubMed
    1. Biochimie. 1980;62(7):503-7 - PubMed
    1. Biochem Biophys Res Commun. 1977 Jan 24;74(2):690-8 - PubMed

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