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. 1982 Jan;149(1):407-10.
doi: 10.1128/jb.149.1.407-410.1982.

Amino-terminal sequence of the Tn3 transposase protein

M D Ditto, J Chou, M W Hunkapiller, M A Fennewald, S P Gerrard, L E Hood, S N Cohen, M J Casadaban

Amino-terminal sequence of the Tn3 transposase protein

M D Ditto et al. J Bacteriol. 1982 Jan.

Abstract

The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.

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References

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