Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Dec 11;9(23):6339-50.
doi: 10.1093/nar/9.23.6339.

The actin gene in yeast Saccharomyces cerevisiae: 5' and 3' end mapping, flanking and putative regulatory sequences

D Gallwitz, F Perrin, R Seidel

The actin gene in yeast Saccharomyces cerevisiae: 5' and 3' end mapping, flanking and putative regulatory sequences

D Gallwitz et al. Nucleic Acids Res. .

Abstract

The 5' and 3' flanking regions of the yeast actin gene have been sequenced and the ends of the actin mRNA were determined by the single-strand nuclease mapping procedure. The mRNA starts with a pyrimidine residue 141 (or 140) nucleotides upstream from the initiation codon. The actin gene lacks a typical "TATA" box 30 base pairs upstream from the mRNA start site but it contains a region homologous to the canonical sequence 5'-GGCTCAATCT-3' which is found in several eukaryotic genes 70 to 80 bp upstream from the mRNA cap site. Judging from the S1 nuclease mapping, there are two populations of actin mRNA terminating 98 and 107 nucleotides downstream from the stop codon. The 3' termini are preceded by three AATAAA sequences found in most eukaryotic polyadenylated mRNAs.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1976 Apr 2;425(4):401-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4461-5 - PubMed
    1. Nature. 1976 Sep 16;263(5574):211-4 - PubMed
    1. Cell. 1977 Nov;12(3):721-32 - PubMed
    1. Nucleic Acids Res. 1978 Jul;5(7):2359-71 - PubMed

Publication types

Associated data