An ultrastructural study of the effects of asbestos fibres on cultured peritoneal macrophages

Abstract

The effects of crocidolite and chrysotile fibres on lavaged peritoneal macrophages have been studied by both scanning (SEM) and transmission (TEM) electron microscopy. SEM provided little information (as the surface topography did not reflect the underlying cytoplasmic organization) except that it showed that individual macrophages often partially engulfed many long fibres in a random fashion. TEM revealed the fibres in and protruding from membrane-bound vacuoles, free in the cytoplasm and penetrating the nucleus. The cellular distribution of the fibres is discussed in terms of the cytotoxic nature of the fibres and their ability to produce a selective release of enzymes from the macrophages.