Instability of the viral M protein in BHK-21 cells persistently infected with Sendai virus

Abstract

The study of viral protein expression in BHK cells persistently infected with Sendai virus showed that the viral M protein was greatly reduced in amount or absent in these cells. Pulse-chase experiments demonstrated that the M protein was synthesized at a normal rate, but was unstable compared to the other viral proteins. The M protein instability was independent of temperature and could account for part of the reduction in viral production by persistently infected cells. When a virus stock was grown in embryonated chicken eggs from viruses produced by persistently infected BHK cells, the M protein of this stock presented a restored stability in BHK cells.