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. 1982 Apr;150(1):410-3.
doi: 10.1128/jb.150.1.410-413.1982.

Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans

L A Sherman, P van de Putte

Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans

L A Sherman et al. J Bacteriol. 1982 Apr.

Abstract

A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicated in both organisms, had unique sites for two restriction endonucleases, conferred ampicillin resistance on both organisms, and could be used as a cloning vector in A. nidulans.

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References

    1. J Biol Chem. 1979 Feb 10;254(3):905-10 - PubMed
    1. Mol Gen Genet. 1980 Apr;178(1):203-11 - PubMed
    1. Adv Microb Physiol. 1979;20:1-102 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 - PubMed
    1. J Bacteriol. 1978 Oct;136(1):414-8 - PubMed

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