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. 1982 Apr;150(1):429-32.
doi: 10.1128/jb.150.1.429-432.1982.

Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion

B L Wanner, J Bernstein

Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion

B L Wanner et al. J Bacteriol. 1982 Apr.

Abstract

A phoR strain was constructed with transposon Tn10 inserted near the phoM+ locus. This was done without any prior knowledge of the phoM map location. Subsequently, we defined the phoM map position by screening tetracycline-sensitive (Tcs) derivatives for mutants which were both alkaline phosphatase negative (ther phoR phoM double mutant phenotype) and auxotrophic simultaneously. Some of these mutants were Thr-. Bacteriophage P1-mediated transductions were used to confirm that phoM and its nearby Tn10 insertion were closely linked to thr. Unexpectedly, 7 of 10 mutants analyzed also had mutations unlinked to the phoM-thr-Tn10 region. These may represent a new type of Tn10-promoted molecular event which is caused by transposition of a Tn10 end (IS10).

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