Internal promoters of the rpoBC operon of Escherichia coli
- PMID: 6278264
- DOI: 10.1007/BF00352538
Internal promoters of the rpoBC operon of Escherichia coli
Abstract
Four ribosomal protein genes, rplA, rplJ, rplK and rplL form an operon in E. coli together with the genes rpoB and rpoC which encode the beta and beta' subunits of RNA polymerase. Transcription is initiated principally at two promoters, PL11 and Pl, the overall structure of the operon being (in the direction of transcription) PL11 rplK rplA Pl rplJ rplL rpoB rpoC. Here we describe studies of phage lambda derivatives carrying various segments of this operon, which demonstrate the existence of at least three additional weak internal promoters, and help to define their positions and strengths.
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