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. 1982 Jan 22;10(2):719-33.
doi: 10.1093/nar/10.2.719.

Reduced-stringency DNA reassociation: sequence specific duplex formation

H E Burr, R T Schimke
Free PMC article

Reduced-stringency DNA reassociation: sequence specific duplex formation

H E Burr et al. Nucleic Acids Res. .
Free PMC article

Abstract

Reduced-stringency DNA reassociation conditions allow low stability duplexes to be detected in prokaryotic, plant, fish, avian, mammalian, and primate genomes. Highly diverged families of sequences can be detected in avian, mouse, and human unique sequence dNAs. Such a family has been described among twelve species of birds; based on species specific melting profiles and fractionation of sequences belonging to this family, it was concluded that permissive reassociation conditions did not artifactually produce low stability structures (1). We report S1 nuclease and optical melting experiments, and further fractionation of the diverged family to confirm sequence specific DNA reassociation at 50 degrees in 0.5 M phosphate buffer.

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References

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