Epidermal growth factor and insulin cause normal chicken heart mesenchymal cells to proliferate like their Rous sarcoma virus-infected counterparts

Abstract

Normal chicken heart mesenchymal cells at low culture density are proliferatively quiescent in a physiological culture medium containing heparinized, heat-inactivated, chicken plasma at 10%. Rous sarcoma virus-infected chicken heart mesenchymal cells, on the other hand, proliferate maximally in this same medium, undergoing a 60-fold increase in cell number during 4 days of exponential growth. When normal heart mesenchymal cells are cultured for 4 days in the presence of epidermal growth factor at 1 micrograms/ml they undergo a 16-fold increase in number, with graded responses to lower concentrations of the factor. In the presence of insulin at 10 micrograms/ml, normal heart mesenchymal cells multiply by a factor of 3 over a 4-day period. The addition of epidermal growth factor (1 microgram/ml) and insulin (10 micrograms/ml) to cultures of normal chicken heart mesenchymal cells causes these cells to proliferate at a rate comparable to that of their RSV-infected counterparts.