Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976;12(1-2):171-81.
doi: 10.1016/0022-1759(76)90107-1.

The covalent coupling of proteins to periodate-oxidized sephadex: a new approach to immunoadsorbent preparation

M B Wilson, P K Nakane

The covalent coupling of proteins to periodate-oxidized sephadex: a new approach to immunoadsorbent preparation

M B Wilson et al. J Immunol Methods. 1976.

Abstract

Proteins were covalently coupled in good yield to periodate-oxidized Sephadex. The reaction was studied in detail with respect to Sephadex type, sodium m-periodate concentration, protein (albumin or immunoglobulin) concentration, time course, temperature dependence, and pH optimum. The complexes were stable and were used successfully for the immunoadsorption of sheep anti-rabbit immunoglobulin antibodies.

PubMed Disclaimer

Publication types

LinkOut - more resources