Production of a T-antigen-related protein in mammalian cells after stable transformation with a cloned SV40 gene fragment
- PMID: 6281154
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Production of a T-antigen-related protein in mammalian cells after stable transformation with a cloned SV40 gene fragment
Hoppe Seylers Z Physiol Chem.
1982 Apr.
Abstract
A recombinant plasmid based on pBR322 has been constructed which carries the replicator proximal early region of SV40 DNA, including the viral origin of replication (ORI). It lacks a major part of the tumour antigen 3'-coding region, the large T-antigen termination codon and the polyadenylation site. The recombinant plasmid was transferred together with the herpes simplex virus thymidine kinase gene, as a selectable marker into mouse LTK- cells. Integration and expression of the cloned SV40 gene fragment in TK+ transformants could be demonstrated by DNA restriction and blot hybridization and by immunofluorescence techniques.
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