Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Jan;128(1):219-22.
doi: 10.1099/00221287-128-1-219.

Generation of a membrane potential by one of two independent pathways for nitrite reduction by Escherichia coli

Generation of a membrane potential by one of two independent pathways for nitrite reduction by Escherichia coli

N R Pope et al. J Gen Microbiol. 1982 Jan.

Abstract

A set of four isogenic Escherichia coli strains has been constructed in which all possible combinations of NADH- and formate-dependent nitrite reductases are active or inactive. Each pathway can be inactivated genetically without a corresponding loss in the other activity: the two pathways are therefore biochemically independent. The generation of a membrane potential during nitrite reduction by formate has been demonstrated using an ion-selective electrode specific for a lipophilic cation. The observed energy conservation results, at least in part, from the ability of formate dehydrogenase in E. coli to pump protons.

PubMed Disclaimer

Publication types