cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin
- PMID: 6283523
- PMCID: PMC346213
- DOI: 10.1073/pnas.79.8.2437
cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin
Abstract
A recombinant plasmid with a cDNA sequence transcribed from mouse skeletal muscle RNA is shown to hybridize with mRNAs for myosin light chains LC1F and LC3F. The inserted fragment corresponds exclusively to the 3'-noncoding region of the mRNA. It hybridizes almost exclusively with the two light chain messengers from fast skeletal muscle RNA of adult mouse. Slight hybridization is seen with RNA from heart muscle and embryonic skeletal muscle. The implications of the conservation of the 3'-noncoding regions between the two mRNAs are discussed.
Similar articles
-
Isolation of repetitive clones from human muscle cDNA library.Biosci Rep. 1986 Jul;6(7):633-8. doi: 10.1007/BF01114757. Biosci Rep. 1986. PMID: 3022839
-
Sequential accumulation of mRNAs encoding different myosin heavy chain isoforms during skeletal muscle development in vivo detected with a recombinant plasmid identified as coding for an adult fast myosin heavy chain from mouse skeletal muscle.J Biol Chem. 1983 Nov 25;258(22):13867-74. J Biol Chem. 1983. PMID: 6196357
-
Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.Proc Natl Acad Sci U S A. 1981 May;78(5):2843-7. doi: 10.1073/pnas.78.5.2843. Proc Natl Acad Sci U S A. 1981. PMID: 6265916 Free PMC article.
-
Cloning of contractile protein genes.Cell Muscle Motil. 1983;3:195-257. doi: 10.1007/978-1-4615-9296-9_7. Cell Muscle Motil. 1983. PMID: 6200200 Review. No abstract available.
-
The cellular basis of myosin heavy chain isoform expression during development of avian skeletal muscles.Dev Biol. 1987 Sep;123(1):1-9. doi: 10.1016/0012-1606(87)90420-9. Dev Biol. 1987. PMID: 3305110 Review. No abstract available.
Cited by
-
The myosin alkali light chain proteins and their genes.Biochem J. 1985 Oct 15;231(2):249-61. doi: 10.1042/bj2310249. Biochem J. 1985. PMID: 3904738 Free PMC article. Review. No abstract available.
-
Myosin light chain-1: genetic analysis of three variants found in fast white chicken muscle and investigation of linkage with the muscular dystrophy gene.Biochem Genet. 1985 Feb;23(1-2):17-27. doi: 10.1007/BF00499109. Biochem Genet. 1985. PMID: 3994657
-
Antisense suppression of skeletal muscle myosin light chain-1 biosynthesis impairs myofibrillogenesis in cultured myotubes.J Muscle Res Cell Motil. 1995 Feb;16(1):45-56. doi: 10.1007/BF00125309. J Muscle Res Cell Motil. 1995. PMID: 7751404
-
Probing myosin light chain 1 structure with monoclonal antibodies.J Muscle Res Cell Motil. 1992 Jun;13(3):329-40. doi: 10.1007/BF01766461. J Muscle Res Cell Motil. 1992. PMID: 1527220
-
Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs.Nucleic Acids Res. 1982 Oct 11;10(19):6099-110. doi: 10.1093/nar/10.19.6099. Nucleic Acids Res. 1982. PMID: 6128725 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases