Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody
- PMID: 6284965
- PMCID: PMC256850
- DOI: 10.1128/JVI.41.3.1099-1104.1982
Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody
Abstract
Glycoproteins gD-1 and gD-2 of herpes simplex virus types 1 and 2, respectively, were purified on an immunoadsorbent consisting of the type-common monoclonal antibody HD-1 linked to Sepharose. Each glycoprotein was of sufficient purity, quantity, and biological activity to be used for immunological and biochemical studies. Each glycoprotein induced high titers of type-common monospecific neutralizing antibody in mice. Amino aicd analysis indicated that gD-1 and gD-2 had similar though not identical amino acid compositions.
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