Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982;185(3):510-2.
doi: 10.1007/BF00334150.

Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli

W Boidol, M Simonis, M Töpert, G Siewert

Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli

W Boidol et al. Mol Gen Genet. 1982.

Abstract

Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment which carried the phoA promoter and by determining the nucleotide sequence of a 160 bp portion of this subfragment comprising the codons for the N-terminal end of pre-alkaline phosphatase. From this DNA sequence the leader sequence of alkaline phosphatase which consists of 21 amino acids was derived.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3369-73 - PubMed
    1. J Bacteriol. 1979 Sep;139(3):932-9 - PubMed
    1. Genetics. 1977 Feb;85(2):203-8 - PubMed
    1. Mol Gen Genet. 1977 Mar 28;152(1):43-51 - PubMed
    1. Microbiol Rev. 1980 Mar;44(1):1-56 - PubMed

Associated data

LinkOut - more resources