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. 1982 May 25;10(10):3133-48.
doi: 10.1093/nar/10.10.3133.

Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29

H M Fried, J R Warner

Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29

H M Fried et al. Nucleic Acids Res. .

Abstract

A cosmid clone bank of yeast DNA has been used to isolate the cycloheximide resistance gene cyh2 of Saccharomyces cerevisiae. A cosmid carrying this gene was identified by cross hybridization to another cloned gene, tsm437. The two genes, which are tightly linked genetically are both present on a 31 kb segment of cloned DNA. The cyh2 gene encodes ribosomal protein L29, a component of the large subunit. Blot hybridization analysis reveals that this gene is present as a single copy in the yeast genome, unlike many other yeast ribosomal protein genes which appear to be duplicated. The cyh2 gene also appears to contain an intervening sequence, a characteristic common to most yeast ribosomal protein genes that have been cloned.

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