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. 1982 Apr 1;203(1):93-8.
doi: 10.1042/bj2030093.

The SalGI restriction endonuclease. Enzyme specificity

A Maxwell, S E Halford

The SalGI restriction endonuclease. Enzyme specificity

A Maxwell et al. Biochem J. .

Abstract

We have analysed the kinetics of DNA cleavage in the reaction between the SalGI restriction endonuclease and plasmid pMB9. This reaction is subject to competitive inhibition by DNA sequences outside the SalGI recognition site; we have determined the Km and Vmax. for the reaction of this enzyme at its recognition site and the KI for its interaction at other DNA sequences. We conclude that the specificity of DNA cleavage by the enzyme is only partly determined by the discrimination it shows for binding at its recognition sequence compared with binding to other DNA sequences.

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