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. 1982 Apr;18(1):39-46.
doi: 10.1016/0378-1119(82)90054-3.

Cloning of the complete human cytomegalovirus genome in cosmids

B Fleckenstein, I Müller, J Collins

Cloning of the complete human cytomegalovirus genome in cosmids

B Fleckenstein et al. Gene. 1982 Apr.

Abstract

Purified virion DNA (155 X 10(6) Mr) of human cytomegalovirus (CMV) strain Ad169 was partially cleaved with restriction endonucleases HindIII and EcoRI and cloned in the respective cleavage sites of cosmid pHC79. A complete gene library was established in a set of clones containing the viral DNA in long overlapping segments. Restriction maps for HindIII (29 fragments) and EcoRI (36 fragments) were constructed from the linkage of cosmid-cloned fragments, from double digestions of cloned DNA, and from blot hybridization of labeled cloned viral DNA with restriction fragments of virion DNA and singly or doubly cleaved cosmid clones.

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