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. 1982 Sep;151(3):1605-7.
doi: 10.1128/jb.151.3.1605-1607.1982.

A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli

D McCarthy, N L Clayton, J K Setlow

A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli

D McCarthy et al. J Bacteriol. 1982 Sep.

Abstract

A new plasmid cloning vehicle (pDM2) was used to introduce a library of Haemophilus influenzae chromosomal fragments into H. influenzae. Transformants of the highly recombination-defective rec-1 mutant were more likely to contain exclusively recombinant plasmids after exposure to ligated DNA mixtures than was the wild type. pDM2 could replicate in Escherichia coli K-12.

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