Comparative Study
doi: 10.1128/mcb.2.5.593-597.1982.
Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers
- PMID: 6287241
- PMCID: PMC369830
- DOI: 10.1128/mcb.2.5.593-597.1982
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Comparative Study
Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers
Mol Cell Biol.
1982 May.
Abstract
The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.
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