3-Aminobenzamide synergistically increases sister-chromatid exchanges in cells exposed to methyl methanesulfonate but not to ultraviolet light

Abstract

3-Aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis, increased baseline sister-chromatid exchange (SCE) frequencies and acted synergistically with the alkylating agent methyl methanesulfonate to induce exchanges in Chinese hamster ovary and SV40-transformed human (GM637) cells. In contrast, 3-aminobenzamide did not affect the frequency of ultraviolet light-induced SCEs. Our data suggest that, in these 2 cell types, synthesis of poly(ADP-ribose) is more important in damage and repair after exposure to an alkylating agent than after exposure to ultraviolet light.